具体内容包括：

　　简要说明研究设计(study design / protocol)，如：回顾(retrospective / review)，前瞻(prospective)，临床(clinical)，动物(animal)，实验(experimental)，活体内(in vivo)活体外(in vitro)，原位(in situ)随访(follow-up)，对照(controlled)，随机(random / randomized)，双盲交叉(double-blind crossover)，人群(population / cohort / migrant)，对比(comparative)，流行病学(epidemiological)等研究性质;

　　详细说明研究对象(subject)情况，包括动物(animal)的性别(sex)、年龄(age)、物种(species)、品种(breed)、生理状态(physiological state); 微生物菌株(strain)、血清型(serotype)及其他区别特性(identity characteristics);随机分组方法(methods of any random assignment of subjects to groups)和选择标准(criteria for admission to study groups);

　　详细说明所用的药物(drug)，激素(hormone)，试剂(reagent)和其他化学品(chemical)的名称、商标(trademark)、生产厂家(manufacturer)及所在地(location);

　　简要说明测定方法(method of measurement)，包括名称，引文和偏差(variations);

　　简要说明统计学分析方法(method of statistic analysis)

　　这部分的陈述程序一般为：研究设计 → 研究对象性质 → 处理 / 干预方法 → 测定 / 观察手段 → 统计分析

　　除指示性说明外，如："病人资料见表1"(Data of the patients are shown in Table 1)"材料与方法"和"结果"两部分一律用过去时表达。

　　示例：A retrospective analysis was conducted of all adults and pediatric patients (age less than 18 years) who received a prolonged course of parenteral nutrition at UCLA Medical Center from January 1976 through December 1983. [研究设计：性质、时间、地点、对象] Criteria for patient selection included a minimum course of a months and development of caculous or acalculous cholecystitis requiring cholecystectomy. From 1976 through 1983 136 patients underwent a prolonged course of TPN at the UCLA Medical Center. Sixty-two of these patients were found to have gallbladder disease. Cholecystectomy was performed in 21 of these patients before the initiation of TPN, and in 35 patients during or after administration of long term TPN. This latter group of 35 patients forms the basis for this report. [选择标准] Specific data regarding age, sex, length of time receiving TPN, underlying disorders, indication for TPN, number of previous operations, preoperative assessment, and the operative biliary procedure, findings and outcomes were all recorded.[研究对象性质]

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